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Spesialis patologi anatomi mendiagnosis penyakit seseorang berdasar pemeriksaan laboratorium. Ada beberapa teknik pemeriksaan di laboratorium patologi anatomi diantaranya pemeriksaan Histologi morfologi jaringan atau Sitologi Morfologi sel.

Sediaan harus dibuat sebaik mungkin agar spesialis dapat melakukan diagnosis yang akurat. Disini akan diuraikan secara singkat teknik sacroma sediaan pemeriksaan sitologi dan pemeriksaan histologi dilaboratorium Patologi Anatomi.

Pada pemeriksaan sitologi yang diperiksa morfologi sel-sel cairan tubuh. Sediaan atau disebut duga preparat dibuat berupa apusan pada objek glass yang diwarani dengan pewarnaan tertentu.

Terutama yang diperiksa adalah detail dari morfologi untuk memeriksa intisel, untuk melihat apakah sel tersebut sel normal, sel noeplasma jinak atau ganas.

Metode ini umumya digunakan untuk pewarnaan Papsmear tapi terkadang ada juga selain papsmear diwarnai dengan metode ini. Preparat yang kering belum difiksasi akan menyebabkan sel-sel rusak. Apabila tempat pengecatan jauh,setelah difiksasi keringkan dan masukkan kewadah yang dapat menjaga keamanan sediaan.

Untuk bahan sputum diambil bagian berwarna dan kental untuk dibuat pulasan. Bagian yang lain bisa gunakan sebagai sel blog. Tahap periksaan dimulai dari penerimaan sampel di tata usaha. Petugas penerima harus mengecek kembali sampel tidak boleh asal terima. Pada tahap ini dokter juga akan memotong jaringan yang dicurigai. Tahapan prosessing jaringan yaitu, Fiksasi, Dehidrasi, clearing, dan infiltrasi paraffin. Untuk mempertahankan struktur sel sehingga menjadi stabil secara fisik dan kimiawi dan mencegah terjadi dialysis atau pembengkakan pada rupture.

Menarik keluar kadar alcohol yang berada dalam jaringan, memberi warna yang bening pada jaringan dan juga sebagai perantara mesuknya kedalam paraffin. Mengisi rongga atau pori-pori yang ada pada jaringan setelah setelah ditinggal cairan sebelumnya xylol.

Agar mudah dipotong menggunakan mikrotom untuk mendapatkan irisan jaringan yang sangat tipis sesuai yang diharapkan. Ganti etiket dengan yang permanen.

Objek glass jangan diolesi albumin gliserin karena biasanya albumin bila diinkubasi akan mengeras. A method for preparation of frozen sections. I find that I always get my best quality section with a new sharp blade.

[NATURAL] Kista Sarcoma Filodes ll Adalah

Your patients surgery is costing thousands of dollars. Hundreds of dollars are spent on disposables including lap pads, gloves, sponges, drapes, cautery, needles, needle magnets, BP cuff, IV tubing, …….

We are conserving pennies on what may be the most important decision impacting on the procedure. In my practice I treat every patient to a new section of blade. I will change it as soon as my section quality begins to fall. Some tissues such as tough collagenous tissues or calcified tissues will quickly dull the blade. If you cut yourself on a blade that has been used for days, it is like sleeping with numerous partners……without the fun!

I always sit on a stool when I cut. Why would you want to do this hunched over with you neck hyper extended? This position is fine if you are bending over to look in a hole in fear of an animal jumping out at you! But for cutting a frozen section you want to be relaxed and comfortable so that you will have maximum control in your left hand. I believe everyone must first learn to be good with a brush. I consider starting a student on the anti-roll devise like putting a child on crutches before they learn to walk.


The purpose of the brush is to grab and maneuver the section across the stage. The unless you have perfect temperature, a cold section will by nature trying to curl up and pull away from the brush. For this reason I use a brush with stiff bristles and a fairly wide gripping surface. I have found Chinese boar bristles to be the stiffest and work the best for me.

I never understood why anyone would want to use the flimsy camel hair kosta. The section can easily pull away from these flexible hairs. I am now making these brushes availablefor anyone who would like to try them. Hold the brush like a pen in the left hand and stabilize the hand by gently resting the side of the fifth finger on the stage or where ever you can find a place depending on your hand size and cryostat. This gives the operator great dexterity and allows for conservation of movement.

Focus on developing your dexterity so you can control the brush like a fine instrument. Could you catch a snowflake as it is falling? I cut the brush at an angle which approximates the angle I hold the brush in my hand.

Turn the wheel in a continuous uniform motion without hesitation. I have seen many frozen sectionists using a brush stop at the beginning of the section, slowly grab the tissue and then start to turn the wheel. In my experience this practice adds to potential artifacts at the beginning of the section, potential variations in thickness, and leads to difficulties when approaching tissues containing fat. With practice, by holding the brush as I described, the operator is capable of grabbing the tissue in a continuous motion, which began before the tissue meets the knife and continues through the complete section.

As the block begins to move toward the knife the brush moves downward in pace with the block. It is the downward movement of the brush kosta allows you to keep a continuous motion as you grab the section.

It is like handing off a baton in a relay race. The second runner must run along side the first runner for the handoff. The baton never slows down. If the second runner was stopped the first runner would have to slow to a stop and the second runner would have to accelerate with the baton again. As the first few millimeters of the section passes the knife there will be some degree of curling of the section.

It is important to pull the tissue toward you rather than to press it to the cryostat stage. Pressing tissue saecoma the cryostat stage sometimes result in adhesion of the tissue warcoma the stage, especially if the tissue is fatty. This will result in a smeared section and a need to clean the stage. This motion of grabbing and guiding the tissue is like pulling a blanket over you in bed.

The continuous repeated sectioning of a block becomes like turning the pedals of a saarcoma. Both hands are circling in synchrony.

Tissue can be picked up from the cryostat stage or from the block. I routinely pick sections up from the stage.

[NATURAL] Kista Sarcoma Filodes ll Adalah | zombieinfestationbr

When the section is complete the tissue can be picked up by holding the slide just above the section and angle the slide down to touch a portion of the tissue. This extra medium allows a margin of error for curling or flipping at either end before it involves the tissue. If I am having particular difficulties with the section I can stop the section before the last 2 mm.


Occasionally when faced with a difficult situation I may have more luck retrieving the section from the block. This can sometimes offer a solution to problems arising from curling or fat sticking to the stage. Some operators prefer this technique for the majority of their sections. To retrieve from the block the tissue is cut through and stopped when the handle of medium on the far side of the tissue is reached.

At this point the crank is moved backward and the block is reversed away from the knife. The section is uncurled downward with the brush over the face of the block and the section is picked up off the block face rather than the stage.

As I mentioned earlier I hold the slide in my right hand as I turn the wheel. The moment the section is complete, I immediately pick up the tissue on the slide and in a moment it is placed into fixative.

Have your fixative opened in an immediately reachable location. Start with the slide in your hand. If there is delay in fixing the tissue there will be significant drying artifact.

In my experience when the frozen section is sitting cold on the stage the effect of drying is minimal. From the time the tissue touches a warm slide it starts to under go significant drying artifact with loss of nuclear detail and leakage of fluids from the cytoplasm.

The differences are striking. It also demonstrates the quality of cytology possible by frozen section using this system. Thickness of the section. For general surgical pathology I recommend cutting at six microns. This thickness will give a rich stain which is easier to interpret at scanning powers of 2x and 4x where pathologists gather much of their information.

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Very thin sections will often look pale at these powers and fine details are easy to miss. A six micron section will afford a moment more time to avoid drying artifact.

Specialized situations may call for thinner or thicker sections. Thickness must be confirmed visually. In my experience kita will not fllodes cut perfect six micron sections unless all conditions are correct and the sections are being cut repeatedly in uniform motion. The change of surface temperature of the block resting between sections will result in warming and expansion and a thicker section will be cut. This is often followed by a very thin section.

When warmed with the hand the first section will often be thicker. When cutting I always let the first two sections pass then continue on to take to the next section if it appears to be the correct thickness. This is another reason why we want to become skilled with the brush so that we can continuously cut the sections until we are satisfied that we have a section of the correct thickness without other artifacts.